Background: Andrographis paniculata (Burm. F.) Wall ex. Nees, belonging to the family Acanthaceae produces a group of diterpene lactones, one of which is the pharmaceutically important andrographolide. Hypothesis: It is known to possess various important biological properties like hepatoptroective, anticancer, anti-HIV, anti inflammatory, etc. Methods: The production of andrographolide in the cell suspension cultures of Andrographis paniculata by elicitation was investigated. Results: Arachidonic acid enhanced the andrographolide content in the suspension cultures of Andrographis paniculata. The maximum andrographolide production was found to 109.6 0.62% w/w over the control cell cultures 26.40.79% w/w with the addition of arachidonic acid (100 M) on day-7. Conclusion: This study could pave the way for establishing a continuous and higher production of andrographolide, which has an enormous pharmacological importance.